Figure 3.

Sulforaphane (SFN) activates Nrf2 signaling in human trabecular meshwork cells (HTMCs). HTMCs were treated with SFN (20 μM) for 6 hours, followed by the stimulation of H₂O₂ (100 μM) for 24 hours. mRNA levels and protein expression of Nrf2 and phase II antioxidative enzymes NQO-1, HO-1, GCLC, and GCLM (A, B). Data were expressed as mean ± standard deviation. * P<0.05, Control versus H₂O₂, ^# P<0.05, H₂O₂ versus H₂O₂+SFN. Nrf2 – nuclearfactor-E2-related factor 2; NQO-1 – NAD(P)H: quinone oxidoreductase 1; HO-1 – heme oxygenase-1; GCLC – glutamate-cysteine ligase catalytic subunit; GCLM – glutamate-cysteine ligase modifier subunit; H₂O₂ – hydrogen peroxide.