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. 2019 Feb 1;33(3-4):150–165. doi: 10.1101/gad.320481.118

Figure 7.

Figure 7.

KRAS-driven human lung cancer cells with mutant LKB1 require autophagy to support proliferation and survive starvation. (A) Western blot of ATG7, LC3, p62, and β-actin. (B) Clonogenic survival assay of human lung cancer cells in HBSS starvation. (C) Relative cell proliferation of human lung cancer cells without or with ATG7 knockdown in nutrient-rich RPMI medium. (D) Clonogenic survival assay of human lung cancer cells without or with ATG7 knockdown in HBSS starvation. (E) Clonogenic survival assay of LKB1 mutant human lung cancer cells without or with ATG7 knockdown in HBSS starvation supplemented with 2 mM glutamine or 20 µM BSA or BSA-Pal. (F) Basal OCR and relative OCR of LKB1 mutant human lung cancer cells without or with ATG7 knockdown after 1 h of treatment with RPMI or HBSS supplemented with 20 µM BSA or BSA-Pal. (G) Model of autophagy in supporting LKB1-deficient lung tumor growth. For all of the graphs, the error bar indicates ±SEM. (*) P < 0.05; (***) P < 0.001. See also Supplemental Figure S7.