Figure 2.

Loss of ZNRF3 induces rapid adrenal growth. (A) Whole adrenals from Znrf3 cKO and Rnf43;Znrf3 double-knockout (dKO) mice are significantly larger in size at 6 wk compared with control or Rnf43 cKO mice. Bars, 1 mm. (B) Normalized adrenal weights shown as mean and 95% confidence interval (CI). Statistical analysis was performed using Welch's one-way ANOVA followed by Games-Howell post hoc test. (**) P < 0.01; (***) P < 0.001. (C,D) Loss of ZNRF3 disrupts normal adrenocortical architecture, as shown by H&E (C) and IHC (D) for the vascular marker CD31. Bars, 100 µm. All data shown are from female mice.