Figure 5.

CRISPR/Cas9 Nf1 gene editing does not alter K+ currents. Representative I_Ks (A) currents recorded from a Nf1 edited sensory neuron that exhibited rapid activation with little time-dependent inactivation. The peak (B) and steady-state (C) I_Ks in sensory neurons transfected with pSpCas9(BB)-2A-green fluorescent protein (GFP) plasmid containing either control sgRNA or Nf1 sgRNA are not different. The steady-state values were measured at the end of the voltage step. The conductance–voltage relations for the peak (D) and the steady-state (E) measurements. The points in (D) have been fitted by the Boltzmann relation and are shown as the continuous lines. The values in each panel of (D) and (E) represent the mean ± SEM obtained from 11 control sgRNA and 14 Nf1 sgRNA. (F) The steady-state inactivation of I_Ks in neurons from 11 control sgRNA and 14 Nf1 sgRNA. The steady-state inactivation voltage protocol consisted of a step to 160 mV after prepulses to −100 mV. Currents were normalized to the maximal value of G obtained for the −100 mV prepulse. The data points have been fitted by the Boltzmann relation and are shown as a continuous line. Representative I_KA (G) currents recorded from a Nf1 edited sensory neuron. The peak (H) I_KA in sensory neurons transfected with pSpCas9(BB)-2A-GFP plasmid containing either control sgRNA or Nf1 sgRNA are not different. The conductance–voltage relations for the peak (I). The points in (I) have been fitted by the Boltzmann relation and are shown as the continuous lines. (J) The steady-state inactivation of I_K in neurons from 10 control sgRNA and 11 Nf1 sgRNA. The steady-state inactivation voltage protocol consisted of a step to 160 mV after prepulses to −100 mV. Currents were normalized to the maximal value of G obtained for the −100 mV prepulse. The data points have been fitted by the Boltzmann relation and are shown as a continuous line.