Figure 3.
CaM/AC8 FP HTS Screening Results. (a) Primary screening results of 1018 FDA-approved small molecules against CaM/AC8-Nt identified 9 compounds that inhibit the assay >10 SD (32% inhibition). Three compounds later determined to be optically interfering with assay or have unfavorable structures are shown in gray. CaM inhibitor TFP, a member of the screening library, is also highlighted. (b) Screening result for CaM/AC8-C2b identified four compounds that inhibit the assay >10 SD (24% inhibition), two of which also inhibit CaM/AC8-Nt and two of which interfere with assay. Numbers shown correspond to chemical structures in Figure 1. Screening results are summarized in Table 1.