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. Author manuscript; available in PMC: 2019 Feb 5.
Published in final edited form as: Nat Med. 2017 Oct 16;23(11):1277–1286. doi: 10.1038/nm.4421

Figure 2.

Figure 2

CXCR5 expression on NK cells and phenotyping of CXCR5+ NK cells in AGMs. CXCR5 was measured on cells from six chronically infected AGMs and six chronically infected macaques. (a) Gating strategy used to analyze CXCR5 expression on B cells, CD8+ T cells and NK cells. Cells from macaques are plotted in gray, and those from AGMs are plotted in purple. Representative plots are shown. (b) Frequencies of CXCR5+ cells for a given cell population in LNs from distinct tissues. pLN, peripheral lymph node. (c) Phenotypes of CXCR5− and CXCR5+ NK cells from LNs in AGMs. A representative dot plot for one animal is shown. (d) Frequencies of CXCR5+ and CXCR5− NK cells expressing given markers in AGM LN. (e) NK cell subset frequencies in LNs from AGMs and macaques. Individual values for AGMs are shown as purple circles and for macaques are shown as gray squares. The squared and circled regions in the dot plots represent those cells which have been considered for the subsequent analysis. The accompanying numbers indicate the percentage of these cells. A nonparametric Mann–Whitney U-test was applied in d. *P < 0.05, **P < 0.005. Horizontal bars indicate median values, and error bars indicate standard deviation.