Figure 3.

Viral load and NK cells in spleen from chronically infected AGMs and macaques. Ten monkeys per species were analyzed. (a) Cell-associated SIV DNA and RNA levels in spleen. (b) CXCR5⁺ NK cells. Representative dot plots for five of ten randomly chosen monkeys (alphabetical order) are shown. The left and right boxed areas represent the CXCR5⁻ and CXCR5⁺ NK cells, respectively. The accompanying numbers in red indicate the percentage of cells in each box. (c) Cell-associated viral DNA levels in spleen plotted against the frequencies of splenic NK cells in macaques (left) and AGMs (right). (d) Frequency of NK cells among CD45⁺ cells in spleen. (e) Frequencies of CXCR5⁺ cells among NK and CD8⁺ T cells in spleen. (f) Frequencies of CXCR5⁺ and CXCR5⁻ NK cells expressing given markers in spleen. (g) NK cell subsets in spleens from AGMs and macaques. Individual values for AGMs are shown as purple circles and for macaques are shown as gray squares. In c, a nonparametric Spearman’s rank-order correlation was performed to determine the relationship between log(SIV DNA) and NK cell frequency for each species. For all panels except c, a nonparametric Mann–Whitney U-test was applied. *P < 0.05, **P < 0.005, ***P < 0.0005. Horizontal bars indicate median values, and error bars indicate standard deviation.