Figure 4. Variations in the kinetics of intracellular Ca²⁺ accumulation in CTL induced by pMHC/NiNLP conjugates with different pMHC density.

Fluo-3 labelled 68A62 CD8⁺ T cells were mixed with cognate pMHC/NiNLP conjugates at indicated concentrations, and changes in intracellular fluorescent intensity were recorded by flow cytometry. NiNLPs were loaded with: (A) 30 (red) and 10 (black) cognate IV9-HLA-A2 molecules; (B) 30 (red) or 10 (black) cognate IV9-HLA-A2 molecules or mixture of 10 cognate IV9-HLA-A2 molecules and 20 non-cognate Tax-HLA-A2 molecules (blue); (C) 30 cognate IV9-HLA-A2 molecules (red), 30 cognate IV9-HLA-A2mut molecules (green) and 10 IV9-HLA-A2mut (violet). Data shown are representative from 2 to 4 independent experiments.