KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-mouse B220 – APCCy7 (clone: RA3-6B2) | Biolegend | Cat#103223; RRID: AB_313006 |
| Rat anti-mouse CD19 – Pacific Blue (clone: 6D5) | Biolegend | Cat#115526; RRID: AB_493341 |
| Rat anti-mouse ckit – APC (clone: 2B8) | Biolegend | Cat#105805; RRID: AB_313214 |
| Rat anti-mouse CD25 – PE (clone: PC61) | Biolegend | Cat#102007; RRID: AB_312856 |
| Rat anti-mouse IgM – PECy7 (clone: RMM-1) | Biolegend | Cat#406513; RRID: AB_10640069 |
| Rat anti-mouse IgD – PerCP5.5 (clone: 11-26c.2a) | Biolegend | Cat#405709; RRID: AB_1575115 |
| Rat anti-mouse CD4 – FITC (clone: RM4-5) | Biolegend | |
| Rat anti-mouse CD8 – APCCy7 (clone: 53-6.7) | Biolegend | Cat#100713; RRID: AB_312752 |
| Rat anti-mouse B220 – Pacific Blue (clone: RA3-6B2) | Biolegend | Cat#103230; RRID: AB_492877 |
| Rat anti-mouse Gr1 – PE (clone: RB6-8C5) | Biolegend | Cat#108407; RRID: AB_313372 |
| Rat anti-mouse TER119 – PerCP (clone: TER119) | Biolegend | Cat#116225; RRID: AB_893637 |
| Rat anti-mouse CD11b – PECy7 (clone: M1-70) | Biolegend | Cat#101215; RRID: AB_312798 |
| AffiniPure Fab Fragment Goat anti-mouse IgM, μ chain specific – FITC | Jackson ImmunoResearch | Cat#115-097-020; RRID: AB_2338618 |
| IgG from rat serum | Sigma Aldrich | Cat#I4131; RRID: AB_1163627 |
| Bacterial and Virus Strains | ||
| NEB 5-alpha competent E. coli | New England Biolabs | Cat#C2987I |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAPI | Sigma Aldrich | Cat#32670; CAS#28718-90-3 |
| Critical Commercial Assays | ||
| FITC BrdU Flow Kit | BD Biosciences | Cat#559619; RRID: AB_2617060 |
| FITC Annexin V Apoptosis Detection Kit | Biolegend | Cat#640932 |
| Deposited Data | ||
| Pro-B cell RNA-sequencing data | This paper | GEO: GSE119422 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Bmi1+/−: FVB.129P2-Bmi1tm1Brn/MvlJ | Maarten van Lohuizen (van der Lugt et al., 1994) | JAX: 024584 |
| Mouse: B1.8+: B6.129P2(C)-Ightm2Cgn/J | Klaus Rajewsky (Sonoda et al., 1997) | JAX: 012642 |
| Mouse: p53F/+: B6.129P2-Trp53tm1Brn/J | The Jackson Laboratory | JAX: 008462 |
| Mouse: Mx1-cre: B6.Cg-Tg(Mx1-cre)1Cgn/J | The Jackson Laboratory | JAX: 003556 |
| Mouse: Bmi1F/+: Bmi1tm1.1Lees/J | The Jackson Laboratory | JAX: 028572 |
| Mouse: mb1-cre: B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ | The Jackson Laboratory | JAX: 020505 |
| Oligonucleotides | ||
| mRNA expression RT-PCR primer pairs, see Table S3 | This paper | N/A |
| Primer pairs for analysis of VDJH joints, see Table S4 | This paper | N/A |
| Software and Algorithms | ||
| FlowJo | FlowJo, LLC | https://www.flowjo.com |
| Prism | Graphpad | https://www.graphpad.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| NCBI IgBlast | NIH | https://www.ncbi.nlm.nih.gov/igblast/ |
| Other | ||
| Poly(I:C) | InvivoGen | Cat#tlrl-picw |
| BD Cytofix/Cytoperm | BD Biosciences | Cat#554714 |
| Zoombie UV Fixable Viability Dye | Biolegend | Cat#423107 |
| Propidium Iodide Staining Solution | BD Biosciences | Cat#556463 |
| M-MLV Reverse Transcriptase | Promega | Cat#M1701 |
| Maxima Reverse Transcriptase | Thermo Scientific | Cat#EP0741 |
| Maxima SYBR Green Master Mix | Thermo Scientific | Cat#K0241 |
| Roche Expand High Fidelity PCR System | Roche | Cat#11732641001 |
| pGEM-T Easy Vector System | Promega | Cat#A1360 |
| RNeasy Micro Kit | QIAGEN | Cat#74004 |
| RNeasy Mini Kit | QIAGEN | Cat#74104 |
| DNAeasy Blood and Tissue Kit | QIAGEN | Cat#69504 |
| SMARTer low-input RNA kit | Clontech | Cat#634938 |