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. 2019 Feb 5;6:190007. doi: 10.1038/sdata.2019.7

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Copyright © 2019, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

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(a) Dependence of the Shannon index from the sample size for V2-V3 and V3-V4 16S rRNA fragments. (b) Dependence of the Shannon relative error from the sample size for V2-V3 and V3-V4 16S rRNA fragments. (c) The per-sample Shannon indices, point bars indicate 95% CI, red line – average value for V2-V3 fragments, blue line – average value for V3-V4 fragments. (d) Dependence of the Simpson index from the sample size for V2-V3 and V3-V4 16S rRNA fragments. (e) Dependence of the Simpson relative error from the sample size for V2-V3 and V3-V4 16S rRNA fragments. (f) The per-sample Simpson indices, point bars indicate 95% CI, red line – average value for V2-V3 fragments, blue line – average value for V3-V4 fragments. V2-V3 fragments 16S rRNA – red pointer, V3-V4 fragments – blue pointer. The encoding of the samples is indicated in Table 1.