Table 3. Summary table of the quantitative data of sample pools for multiplexed sequencing.
| RUN | SAMPLE | C (nM)A | Volume (uL)B | C (pg/uL)C | C (ng/uL)D | C (pg/uL)E | C (ng/uL)F | Library sizeG | Average sizeH |
|---|---|---|---|---|---|---|---|---|---|
| The final concentration was 30 nM in each pool. A: initial concentration of the libraries; B: volume to use in the 30 nM final pool; C: final concentration of the libraries based on Bioanalyzer (BA) measurements; D: final concentration of the pooled libraries determined by BA; E: final concentration of the single libraries measured by Qubit; F: final concentration of the libraries based on Qubit measurement; G: The length of the single libraries were determined by BA; H: The average length of the mixed pools. | |||||||||
| 1 | Y166 | 199.2 | 1.51 | 41654.8 | 6.4 | 68000 | 8.4 | 343 | 356 |
| Y919 | 124.8 | 2.40 | 26722 | 26300 | 358 | ||||
| Y645 | 74.5 | 4.03 | 16024 | 21500 | 366 | ||||
| 2 | Y375 | 58.781 | 5.10 | 12521.73 | 6.4 | 8610 | 4.7 | 357 | 350 |
| Y316 | 57.027 | 5.26 | 11749.08 | 7200 | 338 | ||||
| Y393 | 86.632 | 3.46 | 18817.57 | 17100 | 356 | ||||
| 3 | Br857 | 111.258 | 2.70 | 24428.34 | 6.4 | 30700 | 6.7 | 375 | 369 |
| Br333 | 54.035 | 5.55 | 11475.42 | 13100 | 375 | ||||
| BrA206 | 131.504 | 2.28 | 27907.52 | 19500 | 357 | ||||
| 4 | Y440 | 39.245 | 7.64 | 8191.29 | 6.4 | 13300 | 7.8 | 352 | 363 |
| Y482 | 25.05 | 11.98 | 5482.86 | 5100 | 375 | ||||
| Y558 | 75.605 | 3.97 | 16207.71 | 18000 | 363 | ||||
| 5 | Y331 | 61.145 | 4.91 | 12795.49 | 6.4 | 13500 | 7.2 | 344 | 357 |
| Y724 | 60.31 | 4.97 | 12715.34 | 20500 | 362 | ||||
| Y292 | 147.258 | 2.04 | 31909.64 | 23500 | 365 | ||||
| 6 | Y591 | 103.25 | 2.91 | 22871.87 | 6.6 | 19500 | 7.3 | 376 | 374 |
| Y520 | 60.856 | 4.93 | 12793.51 | 17400 | 357 | ||||
| Y787 | 110.566 | 2.71 | 25104.72 | 28100 | 388 | ||||
| 7 | Y421 | 75.488 | 3.97 | 16289.39 | 6.5 | 14200 | 5.7 | 355 | 353 |
| Y516 | 61.276 | 4.90 | 13104.89 | 11000 | 351 | ||||
| Y532 | 69.348 | 4.33 | 14934.17 | 14300 | 352 | ||||
| 8 | BIB82 | 139.113 | 2.16 | 29501.49 | 6.0 | 27400 | 5.7 | 350 | 330 |
| Y988 | 68.642 | 4.37 | 13380.9 | 12600 | 315 | ||||
| BI370 | 43.857 | 6.84 | 8660.63 | 8200 | 325 | ||||
| 9 | BI269 | 81.736 | 5.51 | 15828.78 | 6.0 | 11800 | 4.4 | 313 | 329 |
| Y426 | 54.168 | 8.31 | 11230 | 8210 | 345 | ||||
| 10 | Y803 | 43.374 | 6.92 | 8970.21 | 6.4 | 6420 | 4.8 | 341 | 358 |
| A438 | 135.98 | 2.21 | 30004 | 22100 | 373 | ||||
| Y880 | 115.546 | 2.60 | 24433.7 | 19200 | 359 | ||||
| 11 | YA243 | 49.12 | 6.11 | 10428.1 | 6.4 | 9450 | 5.7 | 371 | 361 |
| Y915 | 48.499 | 6.19 | 10605 | 8720 | 363 | ||||
| Y967 | 123.886 | 2.42 | 26052 | 25000 | 349 | ||||
| 12 | Y64 | 139.449 | 2.15 | 29738.07 | 6.4 | 30500 | 7.1 | 357 | 357 |
| Y726 | 112.212 | 2.67 | 23744 | 28300 | 357 | ||||
| B168 | 135.267 | 2.22 | 29033.01 | 32100 | 357 | ||||
| 13 | Y872 | 149.778 | 2.00 | 31377.06 | 6.4 | 28100 | 6.5 | 350 | 361 |
| Br708 | 116.782 | 2.57 | 24462.06 | 33500 | 355 | ||||
| Br969 | 100.18 | 2.99 | 21967.36 | 17400 | 377 | ||||
| 14 | YB479 | 127.498 | 2.35 | 27282.84 | 6.3 | 25500 | 6.1 | 356 | 350 |
| YA205 | 61.118 | 4.91 | 12889.7 | 10030 | 354 | ||||
| YB330 | 95.388 | 3.15 | 19830.54 | 23900 | 340 | ||||
| 15 | Y928 | 67.261 | 4.46 | 14431.43 | 6.4 | 14700 | 6.9 | 358 | 356 |
| Y974 | 99.194 | 3.02 | 21249.16 | 24900 | 367 | ||||
| Y78 | 90.024 | 3.33 | 18648 | 19800 | 343 | ||||
| 16 | BI919 | 113.104 | 2.65 | 24609.88 | 6.4 | 24100 | 7.5 | 377 | 360 |
| Y640 | 161.115 | 1.86 | 34615.56 | 48300 | 369 | ||||
| YA762 | 119.56 | 2.51 | 24368.42 | 28200 | 335 | ||||
| 17 | YA626 | 94.57 | 4.76 | 20189.16 | 6.5 | 22400 | 6.6 | 352 | 360 |
| YA29 | 136.8 | 3.29 | 29716.84 | 28000 | 368 | ||||