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. 2019 Jan 29;10:37. doi: 10.3389/fimmu.2019.00037

Figure 2.

Figure 2

Restrictive gene expression of candidate pan-B cell markers in leukocytes from head kidney, a primary lymphoid organ of salmon. Suspension and adherent head kidney leukocytes (HKLs) were collected separately after 72 h in culture and subsequently sorted by flow cytometry based on cell size and granularity (FSC vs. SSC) and then surface IgM expression. Representative dot plot of the HKL subpopulations are shown in (A) with mean percentage of each fraction indicated in the graph. To ensure purity and quality of the sorted HKL subpopulations, expression of several marker genes: igd (B cell subset), mcsfr (macrophage), cd3-z2 (T cells), and cd9 (broad expression) were examined by RT-qPCR assay (B). Upon validation of sorting protocol, gene expression of cd22 and cd79a genes were subsequently determined (C). Each bar represents mean relative expression data from 3 to 4 fish ± SEM. Means with different letters are significantly different (two-tailed t-test with Welch's correction, p < 0.05).