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. 2019 Jan 29;10:37. doi: 10.3389/fimmu.2019.00037

Figure 3.

Figure 3

Restrictive gene expression of candidate pan-B cell markers in leukocytes from spleen, a secondary lymphoid organ of salmon. Freshly isolated splenocytes were sorted based on size and granularity (FSC vs. SSC) and then surface expression of IgM or IgT. Representative dot plot of the SpL subpopulations are shown in (A) with mean percentage of each fraction indicated in the graph. Cells within the lymphoid gate were sorted into IgM+, IgT+, or IgM− IgT− subsets (SpL L IgM+, SpL L IgT+, and SpL L IgM− IgT−, respectively). IgM− IgT− cells outside the lymphocyte gate (SpL nL IgM− IgT) was also collected. Expression of cd22 and cd79a genes were subsequently determined in the different splenocyte subpopulations (B). Each bar represents mean relative expression data from three pooled samples (five fish per pooled sample) ± SEM. Means with different letters are significantly different (two-tailed t-test with Welch's correction, p < 0.05). Due to very low cell frequency, sorted IgT data was obtained from a pool of 15 fish.