Table 1. RNA-seq profiling to evaluate differential gene expression. All samples had the same source (blood sample) and were processed with the following steps: PBMCs isolation, RNA extraction and RNA-seq.

Sample	GEO	Sex	Age	Age of onset	Mutation
All samples included in this study were Italian. All ALS patients had spinal onset and sample collection was performed after 6 months from exordium.
CTRL1	GSE106443	M	48	na	na
CTRL2	GSE106443	M	60	na	na
CTRL3	GSE106443	M	68	na	na
CTRL4	GSE115259	M	38	na	na
CTRL5	GSE115259	F	36	na	na
CTRL6	GSE115259	F	40	na	na
CTRL7	GSE115259	F	40	na	na
ALS-s1	GSE106443	M	66	65	na
ALS-s2	GSE106443	M	63	61	na
ALS-s3	GSE106443	F	61	60	na
ALS-s4	GSE106443	F	70	68	na
ALS-s5	GSE106443	M	56	55	na
ALS-s6	GSE106443	F	55	53	na
ALS-s7	GSE106443	F	56	55	na
ALS-s8	GSE106443	F	83	73	na
ALS-s9	GSE106443	M	66	60	na
ALS-s10	GSE106443	F	58	58	na
ALS-s11	GSE106443	M	68	65	na
ALS-s12	GSE115259	M	86	84	na
ALS-s13	GSE115259	F	68	65	na
ALS-s15	GSE115259	M	71	70	na
ALS-s16	GSE115259	F	69	67	na
FUS-m1	GSE106443	M	49	48	FUS R521C
FUS-m2	GSE106443	F	50	49	FUS P160R
FUS-m3	GSE115259	M	57	56	FUS G302A
SOD1-m1	GSE106443	M	50	49	SOD1 L106F
SOD1-m2	GSE106443	F	74	73	SOD1 G147S
SOD1-m3	GSE115259	F	58	57	SOD1 G93D
TARDBP-m1	GSE106443	F	52	50	TARDBP A382T
TARDBP-m2	GSE106443	M	68	66	TARDBP G357D
VCP-m1	GSE115259	M	51	50	VCP R191Q