Figure 5.

ERRα regulated the transcription of TGFB1 via directly binding to its promoter. HEC1A (A) or ECC (B) cells were treated with XCT-790 (1 µM) for the indicated times, the mRNA expression of TGFB1 was checked by qRT-PCR; (C) Cells were treated with or without 1 µM XCT-790, the luciferase activities of TGFB1 promoter were measured by use of the dual-luciferase assay; (D) HEC1A cells were treated with or without XCT-790 (1 µM) for 2 h, the binding between ERRα and promoter of TGFB1 was measured by ChIP assay. Each experiment has been replicated for three times. **p < 0.01 as compared with the control group.