Figure 6.

ERRα was involved in the self-feedback loop of TGF-β in EC cells. HEC1A or ECC cells were treated with or without 10 ng/ml TGF-β for 24 h, the mRNA of TGFB1 (A) and ERRα (C) was checked by qRT-PCR; the protein expression of TGF-β and ERRα was checked by western blotting (B); (D) After transfected with siNC or si- ERRα-1 for 12 h, the HEC1A cells were further treated with or without 10 ng/ml TGF-β for 24 h, the mRNA of TGFB1 was checked by qRT-PCR; (E) The mRNA expression of TGFB1 in 32 human EC tissues paired with the adjacent non-neoplastic normal tissues was measured by qRT-PCR and normalized to GAPDH. Each experiment has been replicated for three times. * p<0.05, **p<0.01 as compared with the control group.