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Effect of collagen aging on BIK expression and apoptosis. MCF-7 (left panel) and ZR-75-1 (right panel) cells were seeded on plastic or in adult or old 3D type I collagen matrices for 6 days before harvesting the cells. BIK expression was assessed by RT-qPCR (A) and apoptosis was quantified by ELISA (B). Values represent the mean ± S.E.M. of three independent experiments (**p < 0.01, ***p < 0.001, N.S = not significant).
