Figure 6.
Effect of DDR1 silencing on apoptosis in adult and old collagen 3D matrices. MCF-7 cells were transfected with control or DDR1 siRNA. A. DDR1 expression was analyzed by immunoblotting. GAPDH was used as a loading control. C. DDR1-silenced cells were embedded in adult or old 3D collagen matrices at a density of 1 × 105 cells/ml. After 36 hours of culture, the percentage of apoptotic cells was assessed using the Muse® annexin V and dead cell assay kit. Values represent the mean ± S.E.M. of three independent experiments (**p < 0.01, ***p < 0.001, N.S = not significant).