Table 3.

Prevalence of Nonsense Mutations in Predicted Essential Genes of M. tuberculosis

Gene	Gene Size	Essentiality	Nucleotide Position	Change	Polymorphism Type	Protein Effect	Variant Frequency (%)
hemD	1,679	(Griffin et al. 2011)	148	(G)2 → (G)3	Insertion (tandem repeat)	Frame Shift	0.03
			190	–G	Deletion	Frame Shift	0.03
			588	(G)3 → (G)4	Insertion (tandem repeat)	Frame Shift	0.03
			1,488	–C	Deletion	Frame Shift	0.03
cobL	1,173	(Sassetti and Rubin 2003)	376	C → T	SNP (transition)	Truncation	0.03
			743	–G	Deletion	Frame Shift	0.03
			1,103	–A	Deletion	Frame Shift	0.03
			1,150	C → T	SNP (transition)	Truncation	0.03
Rv2228c homolog	1,095	(Griffin et al. 2011)	552	(G)4 → (G)5	Insertion (tandem repeat)	Frame Shift	0.03
			859	–G	Deletion	Frame Shift	0.03
cobC	1,095	(Sassetti and Rubin 2003)	18	(C)3 → (C)2	Deletion (tandem repeat)	Frame Shift	0.1
			589	(G)3 → (G)2	Deletion (tandem repeat)	Frame Shift	0.03
			595	(C)3 → (C)4	Insertion (tandem repeat)	Frame Shift	0.03
			595	(G)2 → (G)3	Insertion (tandem repeat)	Frame Shift	0.03
			792	(C)6 → (C)7	Insertion (tandem repeat)	Frame Shift	0.03
			792	(C)6 → (C)5	Deletion (tandem repeat)	Frame Shift	0.3
cysH	765	(Griffin et al. 2011)	106	–A	Deletion	Frame Shift	0.03
			687	(A)3 → (A)2	Deletion (tandem repeat)	Frame Shift	0.03
che1	846	(Griffin et al. 2011)	5	–C	Deletion	Frame Shift	0.03
			57	AGC → CT	Deletion	Frame Shift	0.1
			59	CA → T	Deletion	Frame Shift	0.03
			60	–A	Deletion	Frame Shift	0.3
			90	–G	Deletion	Frame Shift	0.1
cysG	1,218	(Griffin et al. 2011)	360	–C	Deletion	Frame Shift	0.03
			432	–G	Deletion	Frame Shift	0.03
			891	–C	Deletion	Frame Shift	0.03
cobQ2	696	(Griffin et al. 2011)				not found