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. 2019 Jan 14;10(1):82–96. doi: 10.1080/21505594.2018.1559673

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Serum IgG and mucosal IgA responses against S. Paratyphi A and S. Typhimurium LPS.

The LPS of S. Paratyphi A and S. Typhimurium were used to coat ELISA plates. After a booster immunization, the serum IgG and vaginal wash IgA responses were measured by ELISA. (a) The levels of anti-S. Paratyphi A LPS IgG induced by S1112, S1151 (pSS978) and S1166 (pSS978) were significantly higher than those induced by S738 (***, P < 0.001). (b) The level of anti-S. Typhimurium LPS IgG induced by S738 was significantly higher than that induced by S1112 (***, P < 0.01), while there were no significant differences among the others. (c) Vaginal wash anti-S. Paratyphi A LPS IgA levels induced by S1112, S1151 (pSS978) and S1166 (pSS978) were significantly higher than those induced by S738 (***, P < 0.01). (D) Vaginal wash anti-S. Typhimurium LPS IgA induced by S738 was significantly higher than that induced by S1112 (***, P < 0.01). The negative control groups (BSG) did not mount a detectable immune response in any test. The concentration of antibodies was calculated using a standard curve. All concentrations of the measured samples were within the range of the standard curve. Error bars represent the standard errors of the means.