Figure 4.

Flow cytometry histograms of C3 complement deposition.

The percentage of FITC-positive bacteria was determined by flow cytometry. The wild-type S. Paratyphi A was incubated with the sera from mice that had been vaccinated with S738 (a), S1112 (b), S1151 (pSS978) (c) and S1166 (pSS978) (d). Accordingly, approximately 69.6%, 85.4%, 94.8% and 89.1% S. Paratyphi A were detected positive, respectively. The wild-type S. Typhimurium was incubated with sera from mice that had been vaccinated with S738 (e), S1112 (f), S1151 (pSS978) (g) and S1166 (pSS978) (h). Accordingly, approximately 94.0%, 86.6%, 87.7%, 90.9% of S. Typhimurium were detected positive, respectively. The blue line indicated the test groups mentioned above. The negative control (black line) was the wild-type S. Paratyphi A or S. Typhimurium incubated with naïve mice serum, and the positive control (red line) was the wild-type S. Paratyphi A or S. Typhimurium incubated with O2 or O4 single-factor rabbit antisera.