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. 2019 Jan 14;10(1):82–96. doi: 10.1080/21505594.2018.1559673

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The differential uptake of S. Paratyphi A and S. Typhimurium by RAW264.7 cells after serum opsonization.

Opsonophagocytic uptake of the wild-type S. Paratyphi A and S. Typhimurium by RAW264.7 macrophages was evaluated after treatment with the sera from mice that had been immunized with live attenuated vaccine candidates. (A) In comparison to the control (S. Paratyphi A opsonized with naïve serum), the uptake of S. Paratyphi A opsonized with sera from mice primed with S1112, S1151 (pSS978) and 1066 (pSS978) was significantly higher (**, P < 0.01). (B) The uptake of S. Typhimurium opsonized with sera from mice primed with S738, S1112, S1151 (pSS978) and S1166 (pSS978) was significantly higher than those opsonized with naïve mice serum (**, P < 0.01; ***, P < 0.001). The number of enumerated cells was normalized to 100% as the maximal value. The error bars represented the standard errors of the means.