Figure 1.

Human Intestinal Enteroids Have An Intact Intestinal Epithelium That Can Be Modified By EGTA (A) Representative images of FD4 dynamics in human enteroids. Human enteroids were injected with FD4 and imaged at 0, 5, 10, 20, and 23h postinjection. Control group retained the majority of injected FD4 within the lumen over 12 h. The addition of EGTA to the media 20h after injection resulted in the rapid loss of FD4 from the lumen, indicating the loss of epithelial paracellular barrier function. (B) Quantitation of barrier disruption by determination of the fraction of initial FD4 fluorescence retained over time. Points represent the medians, and bars represent the interquartile ranges. Control human enteroids retained 55% of FD4 over 20h, while the EGTA-treated enteroids retained 7% of FD4 over 20h (p < 0.01* by Mann-Whitney test, compared to control).