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. 2018 Nov 21;70(3):897–908. doi: 10.1093/jxb/ery413

Fig. 2.

Fig. 2.

Defect of guard mother cell (GMC) division in the Arabidopsis asc octuple-mutant. (A) Abaxial epidermis of cotyledons of the wild-type (WT) and acs octuple-mutant. The arrow indicates a single guard cell (SGC). Scale bars are 20 μm (B) E1728 expression in the abaxial epidermis of cotyledons of the wild-type and acs octuple-mutant. The arrow indicates a SGC. Scale bars are 100 μm. (C) Abaxial epidermis of a cotyledon of the acs octuple-mutant. The confocal z-projection image was visualized using propidium iodide staining with a confocal laser-scanning microscope. The yellow arrow indicates a SGC without a central cell wall. White arrows indicate normal stomata with two GCs and a thickened central cell wall. Scale bar is 50 μm. (D) GCs and SGCs imaged with DAPI fluorescence. Sacle bars are 5 μm. (E) Quantitative analysis of DAPI fluorescence intensity in GCs and SGCs. The data are means (±SD) (n=10) and the significant difference between SGCs and GCs was determined using Student’s t-test: *P<0.01. (F) Number of SGCs per cotyledon in the acs octuple-mutant in control (Mock) plants and plants treated with aminocyclopropane-1-carboxylic acid (ACC) and aminoethoxyvinylglycine (AVG). The data are means (±SD) and significant differences compared with the Mock were determined using Student’s t-test: *P<0.01. A total of 50 cotyledons from 25 seedlings were analysed.