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. 2018 Nov 21;70(3):897–908. doi: 10.1093/jxb/ery413

Fig. 6.

Fig. 6.

Aminocyclopropane-1-carboxylic acid (ACC) positively regulates expression of CDKB1;1 and CYCA2;3 in Arabidopsis. (A) Relative mRNA levels of CDKB1;1 and CYCA2;3 (normalized to KAT1) in the wild-type (WT) and fama-1 and flp-1myb88 mutants treated with ACC or aminoethoxyvinylglycine (AVG) compared with the control (Mock). Data are means (±SD) of three biological replicates. Significant differences compared with the corresponding Mock value were determined using Student’s t-test: *P<0.01. Three independent experiments were performed. (B) Expression of pCDKB1;1::GFP and pCYCA2;3::GFP. Representative images are shown. Arrows indicate newly formed GCs. The graphs show quantitative analyses of GFP-positive cells. Data are means (±SD) of three biological replicates. Significant differences compared with the Mock were determined using Student’s t-test: *P<0.01. A total of 50 cotyledons from 25 seedlings were analysed. Three independent experiments were performed.