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In vivo pull-down assay showing interaction between Trx m and STN7/PetC. Protein complexes from Wt and His-tagged o/exTrxm, o/exTrxf, and o/exTrxm-mut cross-linked chloroplasts were pulled-down with Ni-NTA resin. After washing the beads, bound proteins were eluted by boiling and analyzed, together with input fractions, by western blot using anti-STN7, anti-PetC, anti-2-Cys Prx, and anti-Lhcb1 antibodies.
