Figure 1.

Expression of the recombinant TSA-1 in E. coli BL21 (DE3).

A fermenter containing 10L 2xYT medium (supplemented with kanamycin 50 µg/mL) was inoculated with E. coli BL21 (DE3)/pET41TSA1. When the optical density (OD₆₀₀) reached 0.6, the expression of the recombinant protein was induced with 1 mM IPTG (USB) at 30°C maintaining the OD at 30% saturation. A) SDS-PAGE analysis of the induction of rTSA-1 in a batch fermentation. Total cell extract before and after the addition of 1 mM IPTG (2-18h) and without IPTG (N). Molecular marker (M). B) The rTSA-1 expressed as inclusion bodies was purified under denaturing conditions by Ni²⁺ Affinity chromatography. SDS-PAGE analysis of the total cell extract (lane 1), solubilized inclusion bodies (lane 2) and purified rTSA-1 (lane 3).