Figure 4.

WH-mediated stimulation of cytokine release by pDC.

Cytokine levels produced by human pDCs and/or pDC depleted-PBMCs after stimulation with WH, scFv or CpG. Samples were incubated with pDCs of three different donors for 15 minutes at room temperature with the removal of not-bound material (a) or overnight without any washing step (b). The columns represent the mean of replicates with a SD calculated from Graphpad 6.0 software. Donors of the two experiments (a and b) are different and the free CpG was used at molar concentrations comparable to those in the WH preparations. In panel b, the scale of the y-axis is different showing the background secretion of IFNa by pDC depleted PBMCs.