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. 2018 Dec 12;10(1):181–195. doi: 10.1364/BOE.10.000181

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

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Fig. 4 — Above are three frames extracted from a 10 FPS movie from ex vivo lung tissue taken in in non time-resolved modality using the APD detector. Monocytes labeled with Calcein are visible in the foreground. The image intensity represents the voltage output of the APD (in response to fluorescence intensity) on the indicated color scale. The field of view is 500 $μ$ m. Parameters were kept constant throughout the image frames giving good contrast of lung tissue, howeversome saturation of brighter features is therefore present (see Visualization 1^{(31.3MB, mp4)} in the supplementary materials for the full-length movie).