Fig. 2.

Raman experiment on a typical skin tissue section. (a) H&E image shows six measured regions of 100 × 100 μm², being represented as empty squares. Scale bar: 500 μm. (b) H&E image of the serial section. (c) Bright-field image. (d) Reflectance confocal images. (e) Raman pseudo-color image generated by k-means. Region 1 and 2 contains BCC (yellow) and dermis (blue), region 3 contains sebaceous gland (yellow) and MgF₂ substrate (blue), region 4 contains hair shaft (yellow) and hair follicle (blue), region 5 contains inflamed dermis (yellow) and dermis (blue), and region 6 contains epidermis (yellow) and MgF₂ substrate (blue).