Visualization 1 (114.3MB, avi)	Time-lapse video of reconstructed mitochondria in living Drp1−/− MEFs (FOV = 133 x 133 µm, volume acquisition rate = 10 Hz). Scale bars, 10 µm (main), 1 µm (insets). Color scale bar, z = −1.0 µm (blue) to 1.5 µm (red).
Visualization 2 (21.5MB, avi)	Zoomed-in, 3D view of the corresponding region (marked with ‘triangle’) in Supplementary Video 1 (volume acquisition rate = 10 Hz).
Visualization 3 (19.8MB, avi)	Zoomed-in, 3D view of the corresponding region (marked with ‘cross’) in Supplementary Video 1 (volume acquisition rate = 10 Hz).
Visualization 4 (471.2MB, avi)	Time-lapse video of reconstructed Golgi-derived membrane vesicles in living COS-7 cells (FOV = 133 x 133 µm, volume acquisition rate = 100 Hz). Scale bars, 10 µm (main), 1 µm (insets). Color scale bar, z = −1.5 µm (blue) to 1.0 µm (red).
Visualization 5 (470.6KB, avi)	Time-lapse video of a reconstructed diffusing nanoparticle suspended in water (FOV = 8.3 x 133 µm, volume acquisition rate = 1000 Hz).