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. 2019 Jan 24;15(1):e1007913. doi: 10.1371/journal.pgen.1007913

Fig 2. A pulse of CUC2 expression is sufficient to induce tooth formation.

Fig 2

(A) Schematic representation of the construct present in the CUC2i line, allowing ethanol inducible expression of RFP-CUC2 in a cuc2-1 mutant background. The AlcR protein is expressed under the control of the CUC2 promoter and specifically binds to the AlcA promoters upon ethanol application. Thus, activated AlcR drives both GUS and RFP-CUC2 transcription. (B) Young leaves of wild type (WT), cuc2-1 treated with ethanol and CUC2i upon mock and ethanol treatment. Induced teeth are indicated by black arrowheads. CUC2i leaves are observed 4 days after treatment and a representative wild-type primordium of similar blade length is shown. (C-D) Tooth aspect ratio (C) and sinus angle (D) dynamics after 8h or 3x8h inductions. Data are mean ± SEM, tooth number n ≥ 11. The black dashed line indicates 72h, a time at which RFP-CUC2 is not detectable anymore following an 8h induction. (E) Dynamics of RFP-CUC2 pattern after 8h or 3x8h inductions in a CUC2i background. The time at which the sample was imaged following the start of induction is indicated. (F) Quantification of RFP-CUC2 fluorescence after single 8h and triple 8h inductions (designated 8h and 3x8h) of the CUC2i line. Each point is the mean ± SD of n = 10 nuclei per sinus. Scale bars: (B) 100μm and (E) 20μm.