Skip to main content
. 2019 Jan 24;15(1):e1007913. doi: 10.1371/journal.pgen.1007913

Fig 4. CUC3 is a local functional relay for CUC2-triggered tooth outgrowth.

Fig 4

(A) Expression of a pCUC3:CFP reporter during leaf development in a wild-type background. (B) Dynamics of pCUC3:CFP pattern in a CUC2i background after an 8h induction. Time following the start of induction is indicated. The white arrowhead points to epidermal pCUC3:CFP signal. Note that the 0h time-point corresponds to an un-induced control. (C) Real-time RT-PCR quantifications of CUC3 in the wild type (WT), cuc2-1 and CUC2i at 0 to 96 hours after an 8h ethanol induction. RNAs were extracted from microdissected leaf margins and CUC3 levels are normalized by EF1α and qREF. Crosses represent individual data points while squares are mean of the different samples, sample number n ≥ 5. (D) Tooth aspect ratio dynamics after and 8h ethanol induction in a CUC2i and CUC2i cuc3-105 background. Data are mean ± SEM (tooth number n ≥ 10). Statistical significance (Student’s test) is designated by * p<0.05, *** p<0.005. (E) Mean contours of 1100μm-long leaf 11, 12 and 13 WT/cuc3-105; CUC2g-m4/CUC2g-m4 cuc3-105; mir164a-4/mir164a-4 cuc3-105. Scale bars: (A) 50μm, (B) 20μm and (E) 200μm.