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. 2019 Jan 24;15(1):e1007913. doi: 10.1371/journal.pgen.1007913

Fig 7. KLUH also acts as a functional relay for CUC2-triggered tooth outgrowth.

Fig 7

(A) Expression of a pKLUH:GFP reporter during leaf development in a wild-type background. (B) Dynamics of pKLUH:GFP pattern in a CUC2i background after an 8h induction. Time following the start of induction is indicated. Note that the 0h time-point corresponds to an un-induced control. (C) Real-time RT-PCR quantifications of KLUH in the wild type (WT), cuc2-1 and CUC2i at 0 to 96 hours after an 8h ethanol induction. Total RNAs were extracted from microdissected leaf margins and KLUH levels are normalized by EF1α and qREF. Crosses represent individual data points while squares are mean of the different samples, sample number n ≥ 5. (D) Tooth aspect ratio dynamics after an 8h ethanol induction in a CUC2i and CUC2i kluh-4 background. Data are mean ± SEM, tooth number n ≥ 41. Statistical significance (Student’s test) is designated by * p<0.05, *** p<0.005. (E) Mean contours of 1100μm-long leaf 11, 12 and 13 WT/kluh-4; CUC2g-m4/CUC2g-m4 kluh-4; mir164a-4/mir164a-4 kluh-4. Scale bars: (A) 50μm, (B) 20μm, (E) 200μm.