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. 2019 Jan 24;15(1):e1007913. doi: 10.1371/journal.pgen.1007913

Fig 8. KLUH can partially compensate for CUC2 activity.

Fig 8

(A-D) Morphometrics of the pCUC2:KLUH construct effects in the cuc2-3 homozygous mutant or (E-H) transheterozygote cuc2-1/cuc2-3. The effects of two independent transformation events (#18 and #19) were characterized. pCUC2:KLUH was homozygous in the cuc2-3 while it was hemizygous in the transheterozygote cuc2-1/cuc2-3. The leaf primordia were between 600 and 1500μm. (A,E) Mean tooth number ± SEM, (B,F) tooth aspect ratio and (C,G) sinus angle are shown. (D) Leaf primordia about 1mm-long of wild type, cuc2-3 and two independent transgenic lines cuc2-3 pCUC2:KLUH. (H). Almost fully grown leaves of wild type, cuc2-3 and two independent transgenic lines cuc2-1/cuc2-3 pCUC2:KLUH. Tooth number n≥19; aspect ratio and sinus angle n≥34. Letters show treatments with no significant difference (p-value<0.01) in one-way ANOVA followed by Tukey’s HSD. Scale bars = 500μm in (D) and 0.5cm in (H).