Fig. 2.

CHD9 binds to methylated H3 peptides in vitro. A: Multiple alighnment of high degree of conservation of chromodomains of CHD proteins. Color scheme refers to the nature of conserved amino acids (e.g., charged and hydrophobic). B: Binding assay analyzed the interaction of rCHD9 with biotinylated H3 methylated peptides was analyzed in vitro by immunoprecipitation with streptavidin beads followed by western blotting with Flag antibodies. C: Competition assay was analyzed on Western blot between K27me3 and K9me2 peptides displaying a preference for binding rCHD9; even in presence of excess of unmodified H3, the competition did not alter the intensity of binding to rCHD9.