Fig. 7.

n-CHD9 is binding and broadly distributed across the ribosomal locus. A: ChIP assays were performed with α-OSB, α-UBF, α-Pol I, α-Pol II, IgG, or no antibody. n-CHD9 interacts with rDNA promoter along with the expected interactions of UBF and Pol I. B: As actinomycin D induces dispersal and segregation of FCs, the association of both CHD9 and Pol I are reduced at rDNA. C: ChIP–chip experiments using either α-OSB or no antibody show an enrichment of CHD9 (red) at both promoter and transcribed regions of rDNA in contrast to the no antibody negative control (black). Both raw and calculated P-value data are shown.