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Tetracycline-inducible systems for in vivo shRNA expression. An rtTA relies on the presence of tetracycline (+Tet), such as doxycycline, for induction of gene or shRNA expression. Expression is turned off in the absence of tetracycline (−Tet). shRNA, short hairpin RNA; rtTA, reverse tetracycline-controlled transactivator; TRE, tetracycline-responsive element.
