Table 1. Addition of alcohol markedly improves the recovery of RNA from urine as assessed by qRTPCR for human actin.
GHCl pH 6.5 | ||||||
---|---|---|---|---|---|---|
Alcohol: | - | 20% ETOH | 20% ISOH | |||
- RT | + RT | - RT | + RT | - RT | + RT | |
Average Ct (± SD) |
31.5 (±.4) | 30.9 (±.3) |
32.0 (±.3) |
28.5 (±.1) |
31.9 (±.1) |
27.9 (±.1) |
ΔCt | 0.6 | 3.5 | 4.0 |
2:1 urine to lysis buffer; GHCl, guanidine hydrochloride; RT, reverse-transcription; Ct, cycle threshold; SD, standard deviation; ETOH, ethanol; ISOH, isopropanol