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. 2019 Feb 5;14(2):e0210813. doi: 10.1371/journal.pone.0210813

Table 1. Addition of alcohol markedly improves the recovery of RNA from urine as assessed by qRTPCR for human actin.

GHCl pH 6.5
Alcohol: - 20% ETOH 20% ISOH
- RT + RT - RT + RT - RT + RT
Average Ct
(± SD)
31.5 (±.4) 30.9
(±.3)
32.0
(±.3)
28.5
(±.1)
31.9
(±.1)
27.9
(±.1)
ΔCt 0.6 3.5 4.0

2:1 urine to lysis buffer; GHCl, guanidine hydrochloride; RT, reverse-transcription; Ct, cycle threshold; SD, standard deviation; ETOH, ethanol; ISOH, isopropanol