Table 2. Use of guanidine thiocyanate outperforms guanidine hydrochloride for purification of nucleic acids from urine as assessed by qRTPCR for human actin.

Chaotropic salt:	3M GHCl + 33% ISOH	3M GuSCN + 33% ISOH
Average Ct	32.4	30.0
(± SD)	(±.4)	(±.1)

1:1 urine to lysis buffer; GHCl, guanidine hydrochloride; GuSCN, guanidine thiocyanate; Ct, cycle threshold; SD, standard deviation; ISOH, isopropanol