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. 2019 Feb 5;14(2):e0210813. doi: 10.1371/journal.pone.0210813

Table 4. One week stability study examining the effects of different preservatives on human urine.

Temperature: 28°C 4°C -20°C -80°C
Preservative: - 25mM EDTA 25mM EDTA + 0.1% NaN3 3M GuSCN
+ 33% ISOH
- - -
Average Ct
(± SD)
35.3
(±.3)
32.8
(±.2)
32.0
(±.1)
30.6
(±.1)
33.7
(±.8)
31.7
(±.5)
31.3
(±.3)

All samples were extracted with the current method.

GuSCN, guanidine thiocyanate; EDTA, Ethylenediaminetetraacetic acid; NaN3, sodium azide; ISOH, isopropanol; SD, standard deviation; Ct, cycle threshold