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. 2019 Feb 4;151(2):174–185. doi: 10.1085/jgp.201711884

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© 2019 Gamal El-Din et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — Size-dependent effects of mutations of Thr206 on the early phase of inactivation. (A) Top left: Representative current traces of Na_VAb/T206A. Cells were held at −180 mV, and 50-ms depolarizing pulses were applied in 10-mV steps from −180 mV to +50 mV. Top right: Comparison of NavAb/T206A inactivation during the pulse (blue) to Na_VAb/WT (black). The 100-ms pulse was applied from a holding potential of −180 mV to −70 mV. Bottom: Representative current traces of Na_VAb/T206A recorded during 500-ms depolarizing pulses from −180 mV to +50 mV. (B) Voltage dependence of Na_VAb/WT and mutants: Na_VAb/WT, V_1/2 = −97 ± 1.4 mV (black circles; n = 7); Na_VAb/T206A, V_1/2 = −121 ± 2 mV (blue; n = 5); Na_VAb/T206S, V_1/2 = −115 ± 1 mV, (green); Na_VAb/T206C, V_1/2 = −95 ± 4 mV (gray; n = 7); Na_VAb/T206V, V_1/2 = −95 ± 0.9 mV (red; n = 8). The activation curves were constructed from I–V curves in which 500-ms depolarizing pulses were applied. (C) Top: Representative current traces showing inactivation kinetics of Na_VAb Thr206 mutants compared with Na_VAb WT. Bottom: Time constants for early voltage-dependent inactivation plotted versus the volume of the amino acid residue at position 206. (D) Peak inward currents measured during each pulse of 1-Hz trains of depolarizations and normalized to the current produced by the first depolarization of the train for Na_VAb/WT (black), T206C (gray), T206S (green), T206V (red), T206G (purple), and T206A (blue). Error bars represent SEM.