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. 2019 Feb 4;151(2):118–130. doi: 10.1085/jgp.201812182

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© 2019 Abbineni et al.

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Figure 10. — Coexpression of CgA does not influence the release of NPY EGFP or PAI Phl. Chromaffin cells were transfected with combinations of plasmids encoding the indicated proteins. Secretion was stimulated by perfusion with elevated K⁺ and monitored using TIRF microscopy at the rate of 36–60 Hz for cells expressing NPY and CgA, and at 60 Hz for cells expressing PAI-Phl. Each data point represents one secretion event. The duration of each event was determined using a custom program written in IDL (described in Materials and methods). The red dashed lines represent the median duration. n = 288 for NPY-EGFP, n = 170 for CgA-EGFP, n = 297 for NPY-EGFP + CgA-HA, n = 162 for PAI-Phl, and n = 107 for PAI-Phl + CgA-HA. A Kolmogorov–Smirnoff test was performed to compare the distributions. n.s, not significant.