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. 2019 Feb 4;151(2):118–130. doi: 10.1085/jgp.201812182

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© 2019 Abbineni et al.

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Figure 3. — Fusion pore lifetime varies depending on the overexpressed lumenal protein. Chromaffin cells were transfected to express NPY, tPA, or CgA (all tagged with EGFP). Transfected cells were stimulated by perfusion with elevated K⁺ and imaged using pTIRF at 8 Hz. The length of time P/S was elevated was calculated as described in Materials and methods for n = 78 NPY-EGFP, n = 20 tPA-EGFP, and n = 60 CgA-EGFP and binned into three categories. A χ² test was performed to compare the distributions. ^a, significant difference between distribution of curvature durations of NPY-EGFP–containing granules relative to tPA-EGFP– or CgA-EGFP–containing granules (P < 0.0001).