Table 1. Fractional occupancy and transition data summary of control recordings after segment sorting.

Occupancy pattern	C	O1	O2	O3	O4	Adjacent transitions	Total no. of events
P4 n = 3	0.016 ± 0.010	0.104 ± 0.079	0.111 ± 0.066	0.330 ± 0.079	0.440 ± 0.158	97.1 ± 2.3%	71,134
P3 n = 2	0.044 ± 0.019	0.126 ± 0.010	0.264 ± 0.039	0.472 ± 0.102	0.095 ± 0.093	92.7 ± 0.3%	315,560
P2 n = 3	0.064 ± 0.041	0.237 ± 0.188	0.448 ± 0.114	0.232 ± 0.122	0.009 ± 0.007	96.3 ± 2.4%	357,792
P1 n = 4	0.192 ± 0.127	0.476 ± 0.048	0.268 ± 0.121	0.044 ± 0.008	0.015 ± 0.004	99.5 ± 0.5%	117,552

Single-channel events recorded in eight cell-attached patches resulted in 12 files with a sufficient number of events for fitting multistate kinetic models. Patterns P1–P4 each represent the highest fractional occupancy in each of the O₁–O₄ levels. Values for open levels with the greatest fractional area are bolded. Transitions to adjacent conductance levels were >90% for all four occupancy patterns. Mean values ± SD represent the sum of events from all patches (n) in that pattern.