Table 3. Charge–voltage and F-V relationship Boltzmann fit parameters for activation and deactivation.
| Qon-V V1/2 (mV) | k | Qoff-V V1/2 (mV) | k | Mode shift V1/2 (mV) | |
|---|---|---|---|---|---|
| WT | |||||
| pH 7.4 | −48.2 ± 0.5 (3) | 12.2 ± 2.4 | −75.5 ± 2.3 (4) | 11.0 ± 0.7 | −27.3 ± 2.4 |
| pH 6.5 | −44.8 ± 5.0 (8) | 12.2 ± 1.0 | −51.8 ± 3.3 (5) | 13.7 ± 0.5 | −7.0 ± 6.0 |
| Fon-V V1/2 (mV) | k | Foff-V V1/2 (mV) | k | Mode shift V1/2 (mV) | |
| L520C | |||||
| pH 7.4 | −44.7 ± 1.0 (4) | 21.3 ± 1.9 | −76.1 ± 3.2 (5) | 8.8 ± 0.5 | −31.4 ± 3.4 |
| pH 6.5 | −42.8 ± 3.7 (5) | 17.3 ± 0.8 | −57.2 ± 1.9 (5) | 15.5 ± 2.7 | −14.4 ± 4.2 |
| D509A/L520C | |||||
| pH 7.4 | −40.9 ± 8.1 (4) | 29.6 ± 2.9 | −47.7 ± 3.7 (3) | 25.8 ± 5.5 | −5.6 ± 8.9 |
| pH 6.5 | −42.7 ± 3.5 (3) | 27.2 ± 0.6 | −49.6 ± 4.4 (3) | 26.8 ± 1.3 | −6.9 ± 5.6 |
Data are expressed as mean ± SEM, with the number of cells in parentheses.