Figure 5.

Selective expansion of P2rx7^−/− Tfh cells upon pristane injection but not conventional immunization. (A) Scheme of the adoptive transfer and immunization experiment; representative plots of donor CD4⁺ and CXCR5⁺ICOS⁺ T cells within transgenic cells recovered from the draining lymph nodes of recipient mice 1 wk after primary immunization and frequency of recovered donor cells within individual mice after primary immunization (10 mice per group) and secondary response (five mice per group; mean ± SEM). (B) Scheme of the adoptive transfer and pristane administration experiment; representative plots of CD90.2⁺ donor CD4⁺ T cells from spleen at 2 mo after transfer, frequency of recovered donor CXCR5⁺ICOS⁺ CD4⁺ T cells within individual mice at 1, 2, and 4 mo after injection of pristane (mean ± SEM). Each dot in graphs represents an individual mouse. Two-tailed Mann–Whitney U test. *, P < 0.05; **, P < 0.01. (C) Correlation between GC B cells and donor Tfh cell frequencies at the indicated time points. The correlation coefficient r and the respective P value were calculated with nonparametric Spearman test. Each dot in graphs represents an individual mouse.