Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 4;216(2):317–336. doi: 10.1084/jem.20171976

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Faliti et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — P2X7-mediated caspase activation and Gsdmd cleavage in Tfh cells. (A) Time monitoring of YO-PRO-1 uptake after stimulation with BzATP in ICOS⁻PD-1⁻ and ICOS⁺PD-1⁺ cells within CXCR5⁺ CD4⁺ T cells from spleen of WT and P2rx7^−/− mice at 33 wk after pristane administration and frequency of responding cells in the two subsets from WT mice (n = 14). (B) Analysis by flow cytometry and statistics of caspase activation in purified WT and P2rx7^−/− Tfh cells upon stimulation with BzATP as indicated (n = 4). (C) Representative Western blot of full-length and caspase-cleaved Gsdmd (FL and Nterm, respectively), and actin on the same cells as in B, either untreated or stimulated with BzATP. A shorter exposure for Gsdm^FL is shown. Histograms show the statistics of Gsdmd^Nterm relative expression normalized on Gsdm^FL in the indicated conditions (n = 3). Bar graphs: mean ± SEM. Two-tailed Mann–Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. ns, not significant.