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. 2019 Feb 4;218(2):474–488. doi: 10.1083/jcb.201807077

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© 2019 Edzuka and Goshima

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Figure 1. — Kinesin-8^Klp67A is a processive plus end–directed motor. (A) Kymograph showing processive motility of kinesin-8^Klp67A-GFP (100 pM) along MTs and accumulation at the MT end. Kinesin-8^Klp67A-GFP enriched at the MT end was first photo-bleached, followed by observation of unbleached kinesin-8^Klp67A-GFP. Horizontal bar, 5 µm; vertical bar, 20 s. (B) Plot of kinesin-8^Klp67A-GFP run velocity, calculated based on kymographs (n = 331). Immobile GFP signals were not counted (the “0” column represents velocity between 0 and 5 µm/min). (C and D) Gliding of stabilized MTs (blue) by the plus end–directed human kinesin-1 motor (K560 construct; nonfluorescent). Rightward motility of MTs indicate that the left end corresponds to MT plus end. Kinesin-8^Klp67A-GFP (green; 4 nM) accumulated specifically at the plus end.